Aim: To observe briefly the animal cell structure and organelles using human check, procedure: a, place a drop of distilled water bat the center of a glass slide. b, smear the tooth pick on the center the center of the glass slide with the distilled water, c, Add a drop of methylene blue solution to the smear. d, cover the glass slide with the cover slip. e, Remove the excess using the cotton wool to wipe the edge of the glass slide. f, Place the prepared ( mount) on the stage of the microscope and observe using with x4, x10, and x40 object lenses respectively and give your observations.?

Qawsw

2 years ago

Observations:

1. **Low Power (x4) Objective Lens:**
- At low magnification, the sample appears relatively large but lacks detail.
- The smear may appear faintly colored due to the methylene blue staining.
- General outlines of cell structures may be visible, but organelles are not clearly discernible.

2. **Medium Power (x10) Objective Lens:**
- As we increase the magnification, more details become visible.
- Cell boundaries become clearer, and some internal structures may start to be distinguishable.
- Nuclei within cells may become more apparent, appearing as dark-stained regions within the cytoplasm.

3. **High Power (x40) Objective Lens:**
- At high magnification, cellular structures and organelles become more defined and observable.
- Organelles such as mitochondria, endoplasmic reticulum, and Golgi apparatus may become visible as distinct structures within the cell.
- Nuclei may reveal finer details, such as nucleoli or chromatin.

Overall, the methylene blue staining aids in highlighting cellular structures by providing contrast between different components of the cell. However, the resolution and clarity of the observed structures may vary depending on the quality of the sample preparation and the microscope being used.