Abstract

The effect of pesticides, chchlorodipheny trichloroethane (DDY) and Benzene hexaxhoride (BHc) on the microflora of the three types of soil (loamy, clay and sandy soil) was conducted. The serial dietitian technique was employed up to 10-3 using nutrient agar, potato dextrose agar and sabourand agar plates. The isolation of both bacterial and fungal organisms were made before treating the soil types with the two pesticides), D.D.T and BHC. and after treating the soil types with D.D.T after treating and BHc. The highest bacterial count before treating the soil types with D.D.T and BHc was got in loamy soil with 133 per ml having PH 7.1, followed by clay soil with 78 per ml and least was sandy soil with 42 per ml. while the highest bacterial count after treating the soil types with D.D.T was gotten in loamy soil 105 per ml, followed by clay soil with 51 and least count was got in sandy with 28 per ml. Also the highest bacterial count after treatment with BHc was obtained in loamy soil with 12F per ml, followed by clay soil with 64 per ml and the least count was got from sand soil with 37 per ml. The highest bacterial isolate from all the soil types before treatment with the two pesticides was pseudomonas with 25 per ml, followed by Bacillus SPP with 24 per ml, and next was achnomycetes with 19 per ml while the least bacterial organism was Rhizobrium SPP. The fungal organisms isolates were Aspergillus species, syncophatastrum species, pernicillum species and mucor species. The results of this project showed that there was reduction in microflora of the soil types after treatment separately with the two pesticides. But the effect was more with D.D.T than with BHc. Also the effects of the two pesticides was highest in sandy soil followed by clay and lastly the loamy soil.

Table of Content

Introduction

1.2 Aims and objectives

1.3 Statement of problem

1.4 Hypothesis

1.5 Limitation of the study

CHAPTER TWO

Literature review

2.1 Pesticide labeling and safety

2.2 Various classes and types of pesticides.

2.3 Effects of pesticide on population of invertebrates in soil

2.4 Effects of pesticides on population of vertebrates in the soil

2.5 Effects of pesticides on population of micro organism in the soil

CHAPTER THREE

3.0 Materials and methods

3.1 Materials

3.2 Method

3.2.1 Sterilization

3.2.2 Collection of pesticides

3.2.3 Collection of three types of soil loamy clay sandy

3.2.4 Preparation of media

2.2.5 Playing technique

3.2.6 Microbial count, staining and microscopic work

3.2.7 Identification of would

3.2.8 Biochemical test for identification

CHAPTER FOUR Result

CHAPTER FIVE Discussion

CHAPTER SIX

6.0 Conclusion and Recommendation

6.1 Conclusion

6.2 Recommendation

References

Appendix

Introduction

INTRODUCTION

According to crupta and Daroren (2003) soil is that portion of the surface of the land which is essential for plant growth. Plant are anchored in the soil by their root, which spread in all direction and which by holding on to the soil keep the plant in position plant draw all their water and most of their food or nutrient from the soil. Soil is therefore the source of food for plants, animals and man. Soil is of different types, sandy, clay and loamy soil. Sandy soil has or size range of 0.2 to 2mm diameter. They are free draining and do not retain any appreciable amount of soil water. It is brown loose and dry. While clay soil range in size from 0.002mm and lower and contains more than 40% of clay particles. They are sticky, plastic and easily moldable into shape. Another type is loamy soil which contains a fair balance of clay , silt and sand particle and it is the best soil for agriculture, as it has a high proportion of organic matter or content soil organic malter represents on accumulation of partially decayed and animal residues. Such material is continually being broken down as a result of the work of soil micro –organism consequently. It is a rather transitory soil constituent and must be renewed constantly by the addition of plant residues. The organic matter content of a soil is small, only 3-5 percent by weight in a representative minerals topsoil. It is a major soil source of two important mineral elements namely phosphorous and suefur and essentially the sole source of nitrogen. Through its effect on the physical condition of soil, organic matter also increase the amount of water a soil can hold and the proportion of water available for plant growth. Further more organic matter is the main source of energy for soil microflora. Without it biochemical activity would come practically to a stand still soil organic matter consist of two groups (a) Original tissue and its partially decomposed equivalent and (b) the humus. The original tissue include the under composed roots and the tops of higher plants. The materials are subject to vigorous attack by soil organism and both plant and animal which use them as sources of energy and tissue building material. The gelatinous, more resistant products of this decompository both the synthesized by the micro organisms and those modified from the original plant tissue are collectively known as HUMUS. This material, usually black or brown in colour is coloidal in nature. Its capacity to hold water and nutrient ions greatly exceeds that of clay, its inorganic counterpart. Small amount of humus thus augment remarkably the soil capacity to promote plant production.

According to Burges and Raw (1967) loamy like clay is a product of degradation and synthesis. And the agencies responsible are the living organisms in the soil both the animal (fauna) and the plant (flora). These organism engineer a myriad of biochemical changes as decay takes place. They also physically chum the soil and help stabilize soil structure. A rest number of organism live in the soil. By far the greater proportion of these belong to plant life. Yet animal are not to be minimized especially in the early stage of organic decomposition.

Edward and lofty in 1969 explain that the activities of specific group of soil organisms are commonly identified by (a) their numbers in the soil (b) their weight per unit volume or area of soil (biomass) and (c) their metabolic activity. Although the relative metabolic activities are not shown, they are generally related to biomass of the organism. As might be expected, the numbers are highest among the micro organism. So great are microflora number that they do minute the biomass in spite of the minute size of each individual organisms. The microflora monopolize the metabolic activity in soil. It is estimated that 60-80% of the total soil metabolism is due to the microflora.

They further said that some of the many naturally and artificially occurring substance have deleterious effect on the life of at least some species of organism in the soil by inhibiting the development of these species. However, there is continuing concern that these chemical substance may also adversary affect various non parasitic segment of the soil microflora. These chemical compound substance are called pesticide. Pesticides are therefore material useful for the control nutigation of animals detrimental to human or economy. Algaecides, deforlant and descant herbicides plant growth regulator and fungicide are used to regulate population of undesirable organisms which compete with or pristine crop or namental plants. Attractant insecticides, muticides, accredits, molluscide, nematodes, repellant and rodenticide are used principally to reduce parasitism and transmission in domestic animals, the loss of crop plant, the destruction of processed food textile wood products as well as parasitism and disease transmission to human.

The Effect Of Pesticides Dichlorodiphenyl Trichloroeyhane (D.D.Y) And Benzene Hexachroride (BHC) On The Microflora Of The Three Types Of Soil.

Key Features

Table of Content

Introduction