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No. of pages: 105
No. of chapters: 5

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Abstract:

Cassava is an important food security crop in Ghana and in the wake of climate change, there is a need for plant breeders to develop varieties with high water use efficiency as well as high photosynthetic rate in order to adapt to the changing climate.

Thus, the photosynthetic rates of eight cassava (Manihot esculenta Crantz) varieties were non-destructively evaluated using photosynthesis meter miniPPM300, from June 2014 to May 2015, with the aim of selecting varieties with high photosynthetic rate for future breeding programmes. The mean photosynthetic rate varied depending on the varieties ranging from 40.5 μmol/m2s in Bosom nsia to 45.2 μmol/m2s in Gbenze.

However, the presence of African cassava mosaic disease (ACMD) marginally reduced the photosynthetic rate to below 40 μmol/m2s in all the varieties. Similarly, the chlorophyll content index (CCI) as measured by chlorophyll meter and spectrophotometer also varied from one variety to another; it was low in Nandom (17.9 CCI) and high in Gbenze (39.93 CCI) using the chlorophyll meter and was also reduced by the presence of the virus.

Although, the stomatal density varied between the varieties it was not influenced by virus infection. Furthermore, ACMD significantly decreased the leaf surface area from 5705.8mm2 in uninfected plants to 1251.6mm2 in infected plants, consequently reducing the number and weight of tubers produced 11 months after planting (MAP).

Molecular Testing of the viruses using virus-specific primers JSP001/JSP002, EAB555F/EAB555R, EACMV1e/EACMV2e at 6 MAP and 11MAP, showed that the mosaic symptoms were caused by African Cassava Mosaic virus disease. Cassava varieties with high photosynthetic efficiency and low virus infection can be used in cassava improvement programmes in Ghana.