Key Features:

No. of pages: 89

Buy Now

Abstract:

This work investigated the antibiotic susceptibility profile of bacteria associated with wound sepsis of patients attending the University of Ilorin, Ilorin, Teaching Hospital. Wound swabs were collected from a total number of Hundred patients with different kinds of wound (surgical wound, burn, ulcers, and pressure sores) and cultured, of which 72 samples showed bacterial growth. Six different species of bacteria were isolated. Staphylococcus aureus (47.2%) Pseudomonas aeruginosa (19.5%), Klebsiella pneumonia (6.85%), Escherichia coli (15.3%), Staphylococcus epidemidis (5.6%) and Streptococcus pyogenes (5.6%).
The antibiotics susceptibility test of these bacterial isolates was performed using the Kirby- bauer dist diffusion method. Gentamycin and Sreptomycin show high effectiveness in all the isolate except Staphylococcus epidermis and Klebsiella with pefloxacin and ceftadine respectively showing sensitivity to them. Resistance was shown amongst Ampicillin, cotrimoxazole, and Tetracycline.
This study has revealed Gentamycin as the only antibiotic to which most bacterial isolates from infected wounds were sensitive. Ampicillin and penicillin were effective against Streptococcus polygenes while zinacef and ceftazidime show high effectiveness against Klebsiella pneumonia.

KEYWORDS: wound infection: antibiotics susceptibility profile: staphylococcus aureus: gentamycin.

Table of Content:

Title Page i

Approval Page ii

Dedication iii

Acknowledgment iv

Table of Content v

List of Figures

List of Table

Abstract

CHAPTER ONE


1.0 Introduction 1

1.1 Problem of Statement 13

1.2 Justification 14

1.3 Aim and Objective of the study 15

CHAPTER TWO


2.0 Literature Review 17

CHAPTER THREE


3.0 Materials and Method 29

3.1 Sterilization of Materials 29

3.2 Sample Size 29

3.3 Collection and Processing Sample 30

3.4 Culture Media 30

3.5 Isolation of Cultures 31

3.6 Characterization of Bacteria Isolates 32

3.7 Colonial Morphology 32

3.8 Cellular Morphology 32

3.9 Biochemical Characterization 34

3.10 Antibiotics Sensitivity Test 36

CHAPTER FOUR


4.0 Results 39

CHAPTER FIVE


5.0 Discussion 60

5.1 Conclusion 66

5.2 Recommendation 67

References





LIST OF FIGURES

Figure 1: Frequency of Occurrence of bacterial from Wound Infection patient in University of Ilorin Teaching Hospital.

Figure 2: Bacterial Isolate from patient with diabetic ulcers in University of Ilorin Teaching Hospital.

Figure 3: Bacterial Isolated from patients with surgical wound in University of Ilorin Teaching Hospital.

Figure 4: Bacterial Isolated from patients with burns in University of Ilorin Teaching Hospital.

Figure 5: bacterial Isolated from patient with pressure sore in University of Ilorin Teaching Hospital.



LIST OF TABLE


Table 1: Morphological Characteristics, Staining Reaction and Biochemical Test

Table 2: Frequency of Occurrence of bacteria from Wound Infection patient in University of Ilorin Teaching Hospital.

Table 3: Bacteria pathogens causing wound infection in patients with Diabetic ulcers.

Table 4: Bacteria pathogens causing wound infection in patients with Surgical Wound.

Table 5: Bacteria pathogens causing wound infection in patients with Burns.

Table 6: Bacteria pathogens causing wound infection in patients with Pressure Sore.

Table 7: Antibiotic susceptibility Patterns of Staphylococcus aureus isolated from wound patients in UITH

Table 8: Antibiotics susceptibility pattern of Pseudomonas aeruginosa isolated from wound in UITH

Table 9: Antibiotic susceptibility pattern of Staphylococcus epidermidis in UITH.

Table 10: Antibiotic Susceptibility pattern of Escherichia coli isolated from wound patients in UITH.

Table 11: Antibiotic Susceptibility pattern of Streptococcus pyogenes isolated from wound patients in UITH.

Table 12: Antibiotic Susceptibility pattern of Klebsiella pneumoniae isolated from wound patients in UITH.