In the Burret test, some protein was mixed with sodium hydroxide solution. Which of the following chemicals should be added to the mixture for a positive results?
Mercurous nitrates
Copper sulphate
Mercuric nitrate
Sodium carbonate
Ammonium hydroxide
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The Biuret test is a chemical test used to detect the presence of peptide bonds in a substance, which indicates the presence of proteins.
Step 1: The sample is first mixed with an alkali, such as sodium hydroxide (
NaOH to create an alkaline environment.
Step 2: A few drops of dilute copper(II) sulphate (
CuSO
) are added.
Positive Result: In the presence of proteins, the copper(II) ions to form a complex with the nitrogen atoms in the peptide bonds. This results in a color change from blue to violet or purple.
Why the other options are incorrect:
Mercuric/Mercurous nitrates: These are used in Millon's test, not the Biuret test.
Sodium carbonate: This is a base but is typically used in the Benedict’s test for reducing sugars.
Ammonium hydroxide: While alkaline, it is not the reagent used to provide the copper ions necessary for the color change in this specific test.
